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Detection of GLK1 protein by affinity purified anti-GLK1 antibodies. Total proteins were extracted from wild-type, glk1, glk2, glk1glk2, and GLK1 overexpressing (GLK1ox) Arabidopsis plants, resolved by SDS-PAGE, and probed with antibodies against GLK1. Asterisks indicate nonspecific proteins detected by antibodies.
