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. 2016 Nov 7;173(1):524–535. doi: 10.1104/pp.16.01546

Figure 5.

Figure 5.

Expression of GFP-GLK1 in the glk1glk2 mutant. A, Representative phenotype of the glk1glk2 mutant transformed with GFP-GLK1 construct. Transformed plants were first grown on MS plates containing Hygromycin B for 2 weeks, transferred to soil, and allowed to grow for another 2 weeks. Wild-type and glk1glk2 plants were grown on MS plates without antibiotics and then transferred to soil. B, Immunoblot analysis of GFP-GLK1. Total proteins were extracted from wild-type, glk1glk2, and transformed plants, and probed with antibodies against GFP. GFP-GLK1 is indicated by an arrow. An asterisk indicates the position of degraded GFP-GLK1. C, Expression analysis of LHCB1 in wild-type, glk1glk2, and transformed plants. The mRNA levels were analyzed by real-time PCR, and expression levels were normalized to that of ACTIN2. Error bars represent 1 se of the mean (n = 3). The expression level in wild-type plants was set to 1. D, Total chlorophyll content in wild-type, glk1glk2, and transformed plants. Error bars represent 1 se of the mean (n = 3). FW, Fresh weight. WT, wild type.