Figure 5.

OsPHO2 modulates the degradation of OsPHF1. A, Protein levels of 3FLAG-tagged OsPHO2 with 4MYC-tagged OsPHF1 in transiently expressed tobacco (Nicotiana benthamiana) leaves. The tag-specific antibodies were used in the immunoblot analysis. The amount of OsPHF1-4MYC protein was reduced when it was coexpressed with 3FLAG-OsPHO2. Coinfiltration of the 4MYC-GFP construct was used as an internal control. B, Immunoblot analysis of OsPHF1 and 3FLAG-OsPHO2 proteins in seedlings of rice wild-type Nip, pho2 mutant and two OsPHO2/pho2 recovery lines. Anti-FLAG and anti-OsPHF1 antibodies were used. The protein staining (bottom) was used as a loading control.