Figure 6.

LO9-177 interacts with OsBC1. A, LO9-177 is able to make a homodimer. It interacts with OsBUL1 and its homologs, such as OsBU1, OsBUL2, and OsBUL3, except ILI1 (OsILI1), with different affinities and with a typical bHLH protein, OsBC1, but does not interact with OsIBH1 and APG. B, GST pull-down assays showed LO9-177:His fusion proteins were pulled down only together with GST:OsBC1 fusion proteins. C, Reconstructed yellow fluorescence from YFPn:OsBC1 and YFPc:LO9-177 was detected only in the nucleus with speckles of transfected rice protoplasts. The negative controls that were conducted by cotransfecting unfused YFP fragments together with a single complementary BiFC interactor protein resulted in no detectable signals (Supplemental Fig. S9C). Bar = 5 µm.