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OsBUL1 interacts with OsBC1 through LO9-177. A, Yeast three-hybrid systems demonstrated that OsBUL1 interacts with OsBC1 only in the presence of LO9-177. However, coexpression of LO9-177 did not affect the interaction between OsBUL1 and APG. B, Schema showing the positive interaction between OsBUL1 and OsBC1 mediated by LO9-177 in yeast cells. C, His pull-down assays demonstrated that OsBC1 can be pulled down with OsBUL1 only in the presence of LO9-177.
