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. 2004 Sep 15;101(39):14067–14072. doi: 10.1073/pnas.0405695101

Fig. 2.

Fig. 2.

DC-mediated transinfection of HCV pseudoviruses into Huh-7 cells. Immature human DC were differentiated from peripheral blood mononuclear cells of two HCV– donors (A and B) by using granulocyte–macrophage colony-stimulating factor and IL-4. (A) Expression of DC-SIGN was quantified by flow cytometry and represented as mean fluorescent intensity (m.f.i.) of an anti-DC-SIGN mAb. DC were incubated with HCV pseudovirus-containing supernatants, washed, and cocultured with Huh-7 cells. Luciferase activity was measured in cell lysates 48 h postinfection. Direct infection of DC by HCV pseudoviruses was measured in the absence of Huh-7 cells (dotted bars). DC were also preincubated with HCV pseudoviruses alone (black bars) or in the presence of anti-DC/L-SIGN 612X (crosshatched bars), mannan (gray bars), or anti-CD81 mAb JS-81 (white bars). Alternatively, JS-81 was added to DC and Huh-7 cells after pseudovirus capture (dotted bars). (B) Alternatively, DC were incubated with HCV pseudovirus-containing supernatants at 37°C (black bars), 4°C (gray bars), or in the presence of chloroquine (white bars), washed, and cocultured with Huh-7 cells. Luciferase activity was measured in cell lysates 48 h postinfection.