Table 1. HCV pseudovirus binding to SIGN+ HeLa cells.

	L-SIGN		DC-SIGN
	p24, pg/ml	Inhibition, %	p24, pg/ml	Inhibition, %
IgG control	121	0 ± 5	160	0 ± 4
mAb 612X	43	59 ± 8	109	30 ± 11
Mannan	23	84 ± 13	49	73 ± 6

L-SIGN+, DC-SIGN+, or parental HeLa cells were incubated with a nonspecific murine IgG (10 μg/ml), anti-L/DC-SIGN mAb 612X (10 μg/ml), or mannan (20 μg/ml), followed by incubation with HCV pseudovirus-containing supernatants. Cell-associated p24 (pg/ml) was measured by using a Coulter HIV-1 p24 antigen assay and corrected for background binding to parental HeLa cells. Percentages of binding inhibition were calculated and adjusted for background by using the formula 100-[(p24 with inhibitors - p24 HeLa with IgG)/(p24 without inhibitor - p24 HeLa with IgG) × 100]%. Negative values are represented as 0 for ease of interpretation. One representative experiment of three independent experiments is shown. The percentages of binding inhibition are means of three independent experiments ± SD.