Table 2. Soluble E2 and HCV pseudovirus binding to primary dendritic cells.

	Fluorescent cells, %	Inhibition, %	Units/ml	Inhibition, %
IgG control	55	—	65,000	—
612X mAb	13	71 ± 15	17,000	74 ± 8
Mannan	22	66 ± 12	32,000	49 ± 12

Primary DC isolated from an HCV- donor were incubated with a control murine IgG (10 μg/ml), anti-L/DC-SIGN mAb 612X (10 μg/ml), or mannan (20 μg/ml), followed by incubation with fluorescent beads coupled with soluble E2. Binding was measured by flow cytometry and expressed as a percentage of fluorescently labeled cells. Alternatively, DC were incubated with HCV pseudovirus-containing supernatants, and the number of cell-associated HIV-1 RNA copies was quantified by real-time PCR. Percentages of inhibition were calculated as described for Table 1. One representative experiment of three independent experiments is shown. The percentages of binding inhibition are means of three independent experiments ± SD.