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β-Gal staining of mouse thymus and spleen. (A) Representative cut tissue pieces of thymus (Upper) and spleen (Lower) from study (S) mice (transgenic) stain deeply blue-green as compared with minimal background staining in controls (C). (B–D) Microsopic analysis of thymus (B) and spleen (C) from transgenic mice reveal the blue-green cytoplasmic staining expected with X-gal, whereas tissues from control mice do not stain (D). Sections are counterstained with nuclear fast red.
