Fig. 1.

Defective differentiation of mo-DCs in patients with XLA. (A) Flow-cytometric analysis of 6-day-old mo-DCs of healthy donors (Upper) and patients with XLA (Lower). Fluorescence was analyzed with a FACScan flow cytometer, and data were processed by cellquest. The percentage of cells that are positive for the indicated markers is shown, and MFIs are indicated in parentheses (representative of six healthy donors and seven patients). (B) Comparison of the percentage of cells expressing CD1a, CD83, CD80, and CD86 in seven patients with XLA (filled circles) and six healthy donors (open circles) after differentiation of DCs for 6 days. The mean values are indicated with a horizontal bar for each marker. Statistical significance as determined by the Mann–Whitney test is indicated.