Fig. 5.

Stimulation of DCs by CD40-reactive NAbs and CD40L operates through distinct signaling pathways. Six-day-old mo-DCs from healthy donors were cultured in the presence of GM-CSF and IL-4 alone (filled bars) or cultured with CD40-reactive NAbs (diagonally hatched bars) or CD40L-transfected fibroblasts (open bars) for 24 h. Nuclear extracts were prepared, and transcription factor profiles in nuclear extracts were detected by ELISA. Data are expressed as the mean ± SD of two experiments. *, P < 0.05, ANOVA.