Figure 5.

Pb apiG3PAT (−) parasites show normal development in the blood and mosquito stages. A. Blood stage growth of Pb apiG3PAT (−) and wild type parasites is indistinguishable in a four‐day in vivo growth assay. Error bars show mean of six mice ± standard error. B. Numbers of exflagellating male gametocytes and mosquito stage parasites in the Pb apiG3PAT (−) and wild type lines are comparable. Exflagellation rate determined per 10⁴ red blood cells (RBCs) immediately prior to mosquito infection, and ookinetes, oocysts and salivary gland sporozoites counted 20–22 h, 14–15 days and 21–22 days post‐infection, respectively. Results from five biological replicates, with mean values indicated by bars.