Table 1. Genes and oligonucleotide primers used in PCR analysis.
| Gene | Primer sequence (5′ to 3′) | Length (bp) | Amplicon size (bp) |
|---|---|---|---|
| GDNF | F: AGACCGGATCCGAGGTGC | 18 | 129 |
| R: TCGAGAAGCCTCTTACCGGC | 20 | ||
| BDNF | F: TACCTGGATGCCGCAAACAT | 20 | 182 |
| R: TGGCCTTTTGATACCGGGAC | 20 | ||
| CNTF | F: ATGGCTTTCGCAGAGCAAAC | 20 | 191 |
| R: CAACGATCAGTGCTTGCCAC | 20 | ||
| GAPDH | F: GTCATCATCTCAGCCCCCTC | 20 | 99 |
| R:GGATGCGTTGCTGACAATCT | 20 |
PCR: polymerase chain reaction; GDNF: glial cell-derived neurotrophic factor; BDNF: brain-derived neurotrophic factor; CNTF: ciliary neurotrophic factor; F: forward primer; R: reverse primer.