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. 2017 Jan 5;7:39732. doi: 10.1038/srep39732

Figure 5. PLA of NUPR1 and MSL1.

Figure 5

Cells were plated on coverslips and transfected with pcDNA3-NUPR1-Flag and pcDNA4-MSL1-V5 constructs. After one day, cells were treated simultaneously with Oxaliplatin (10 μM) to induce DNA damage (thus promoting MSL1-NUPR1 interaction), and (A) DMSO or (B) Compound-15 (in DMSO); PLA was performed 24 h later as described in the Methods section. The 40x magnification was analyzed with ImageJ to count the number of red dots which represent NUPR1/MSL1 binding. The reduction in the number of red fluorescent dots is proportional to the inhibition of MSL1-NUPR1 interaction by Compound-15.