Table 1. Dissociation constants of the NUPR1-Compound complexes, and residues of NUPR1 with NMR-cross-peak broadening affected by binding.
| Compound | Kd (μM)a | Residues | |
|---|---|---|---|
| 1 | Terfenadine | 5.0 | — |
| 2 | Fluphenazine dihydrochloride | 2.0 | Thr68 |
| 3 | Caffeic acid | 2.0 | Ala33; Thr68 |
| 4 | Reserpine | 3.2 | Thr68 |
| 5 | (-)-Isoproterenol hydrochloride | 3.9 | Thr68 |
| 6 | Flunarizine dihydrochloride | 3.1 | Ala33; Thr68 |
| 7 | Halofantrine hydrochlorideb | 3.3 | Thr68 |
| 8 | Levonordefrin | 1.5 | Ala33; Thr68 |
| 9 | (+)-Isoproterenol (+)-bitartrate salt | 4.0 | Ser9; Ala10; Leu29; Ala33; Gly38; Thr68 |
| 10 | Pheniramine maleate | 4.3 | Ser9; His62; Thr68 |
| 11 | Terconazole | —c | Thr68 |
| 12 | Dihydroergotoxine mesylate | 4.0 | Leu29; Leu32; Gly38; Thr68 |
| 13 | Benzethonium chloride | 3.6 | Thr68 |
| 14 | Chlortetracycline hydrochloride | 1.5 | Ala33; Thr68 |
| 15 | Trifluoperazine dihydrochloride | 5.2 | Ala33; Thr68 |
aRelative error is 20%.
bAt the NMR concentrations, the compound precipitated.
cAt the conditions tested, it could not be determined.