Fig. 1. Plasmids used in the dual screen for loss/regain of function.

Both plasmids are circular with critical elements shown in linear format. Both plasmids are shuttle vectors that replicate in both yeast and E. coli (A) The diagram shows the organization of elements important for expression of cre recombinase in pRLS207. (B) The diagram shows the organization of elements in pRR2 used in mutagenisis and cre-mediated recombination. Wild-type amino acids targeted by mutagenesis are shown in red.