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A: RT‐PCR for p15Ink4b and p19Arf mRNA in non‐tumor livers (NT), hepatic tumors (T), a negative control (distilled water: DW) and a positive control (PCR product used as template: PC). A total of 35 cycles of PCR were performed. MW: molecular weight marker. B: p15Ink4b and p19Arf mRNA levels were determined by qRT‐PCR of DEN‐induced hepatic tumors (n = 9) 13 months after neonatal DEN treatment and in age‐matched normal livers (n = 8).
