Table 8. Primers sequences for qPCR.

Name	Protein name	Sequences: Forward (F) and Reverse (R)	Size (bp)	NCBI number
Runx2	Runt-related transcription factor 2	F: 5′-GACAAGCACAAGTAAATCATTGAACTACAG-3′ R: 5′-GTAAGGCTGGTTGGTTAAGAATCTCTG-3′	196	NM_001024630.3
ALP	Alkaline phosphatase	F: 5′-GGACACTGGGCATAGATTTCTCAAG-3′ R: 5′-TGCTGGGATTACAAACACTTCCTTT-3′	109	NM_001632.4
Col1	Collagen type I	F: 5′-GTCACCCACCGACCAAGAAACC-3′ R: 5′-AAGTCCAGGCTGTCCAGGGATG-3′	121	NM_000088.3
Bglap	Osteocalcin	F: 5′-CCTCACACTCCTCGCCCTATT-3′ R: 5′-CCCTCCTGCTTGGACACAAA-3	117	NM_199173.5
Spp1	Osteopontin	F: 5′-GTACCCTGATGCTACAGACG-3′ R: 5′-TTCATAACTGTCCTTCCCAC-3′	139	NM_001040058.1
TRAP	Tartrate-resistant acid phosphatase	F: 5′-TGACAAGAGGTTCCAGGAGAC-3′ R: 5′-GAAGTGCAGGCGGTAGAAAG-3′	178	NM_001111035.1
CTSK	Cathepsin K	F: 5′-AACGAAGCCAGACAACAGATT-3′ R: 5′-CGAGAGATTTCATCCACCTTGTT-3′	170	NM_000396.3
GAPDH	Glyceraldehyde 3-phosphate dehydrogenase	F: 5′-TGCACCACCAACTGCTTAGC-3′ R: 5′-GGCATGGACTGTGGTCATGAG-3′	178	NM_002046.5