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. 2017 Jan 12;2(1):e90861. doi: 10.1172/jci.insight.90861

Figure 1. Lymphatic specificity of potential imaging tracers.

Figure 1

(A) Schematic showing s.c. injection site on dorsal aspect of the paw and location of imaging region of interest (ROI) containing popliteal vein with adjoining collecting lymphatic vessels with mouse in prone position. (B) Representative video frames after s.c. injections of 2% Evans blue (20 μl), a 20-kDa PEG–IRDye680 conjugate (P20D680), and a 40-kDa PEG–IRDye680 conjugate (P40D680) (20 μl of 25 μM each). Uptake of Evans blue into popliteal vein (yellow arrowheads) as well as collecting lymphatic vessels was seen. P20D680 and P40D680 uptake was solely into collecting lymphatic vessels. Each image is representative of n = 3 mice per tracer. (C) Corresponding Prox1-GFP images of regions shown in B. Scale bars: 500 μm. (D) Schematic showing s.c. injection site on dorsal aspect of the paw and location of imaging ROI of the saphenous vein on the contralateral leg with mouse in supine position. (E) Representative video frames showing Evans blue signal in saphenous vein at t = 5 minutes after s.c. injection with no P20D680 or P40D680 signals in saphenous vein at the same time point. Scale bars: 500 μm. (F) Signal enhancement curve over time after s.c. injections of Evans blue, P20D680, and P40D680. n = 3 mice for each tracer. Dashed lines show SD.