Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 5;7:2129. doi: 10.3389/fmicb.2016.02129

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Huang, Chen, Wang, Hsu, Tsai, Liu and Chang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Interaction between Rta and TRIM5α. (A) In a GST-pulldown assay, His-Rta (lanes 3–5) was mixed with GST- (lane 4) and GST-TRIM5α- (lane 5) glutathione-Sepharose beads. Proteins pulled down by the beads were analyzed by immunoblotting (IB) with anti-Rta antibody. Proteins on the glutathione-Sepharose beads (lanes 1 and 2) and Rta in 1% of the lysate (lane 5) were also detected by immunoblotting. (B) Coimmunoprecipitation assay results. Anti-Rta and anti-TRIM5α antibodies were added to the lysate from 293T cells that had been transfected with pLPCX-HA-TRIM5α and pCMV-Rta. Lanes 1 and 5 were loaded with 3% of the cell lysate. Proteins immunoprecipitated (IP) with anti-Rta and anti-TRIM5α antibodies or anti-IgG antibody were detected by immunoblotting (IB), using anti-HA (lanes 1–4) and anti-Rta antibodies (lanes 5–8).