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. 2017 Jan 5;7:2129. doi: 10.3389/fmicb.2016.02129

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Copyright © 2017 Huang, Chen, Wang, Hsu, Tsai, Liu and Chang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reduction of Rta transactivation activity by TRIM5α. 293T cells were cotransfected with pCMV-R, pLPCX-TRIM5α, and (A) pBMRF1, (B) pBMRF1-RRE, or (C) pBMLF1-RRE. Luciferase activities were assessed 24 h after transfection. Each transfection experiment was performed three times, and each sample in the experiment was prepared in duplicate. Rta, TRIM5α, and α-tubulin expressed by the cells were examined by immunoblotting (IB). The data obtained from the reporter assay were subjected to one-way analysis of variance (ANOVA) using SPSS software 12.0. P-values of <0.05 were considered statistically significant. RLU, relative light units.