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. 2017 Jan 5;7:2129. doi: 10.3389/fmicb.2016.02129

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Copyright © 2017 Huang, Chen, Wang, Hsu, Tsai, Liu and Chang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influence of TRIM5α on EBV lytic progression. (A) P3HR1 cells were infected by lentivirus expressing TRIM5α shRNA (shTRIM5α) or control shRNA (Ct-shRNA). Thereafter, cells were treated with sodium butyrate and TPA (SB/TPA) for 48 h, and proteins in the lysates were examined by immunoblotting with anti-Rta, anti-EA-D, anti-TRIM5α, and anti-α-tubulin antibodies. (B) Cells were lysed at 120 h after TPA and sodium butyrate treatment, and the expression of VCA, BFRF3, and α-tubulin was examined by immunoblotting. (C) An EBV lytic DNA replication assay was conducted by qPCR to determine the copy numbers of EBV DNA. The amount of EBV DNA was determined by standard curve, which was in turn established using maxi-EBV DNA purified from E. coli after qPCR analysis. The data were subjected to one-way analysis of variance (ANOVA) using SPSS software 12.0. P-values of <0.05 were considered statistically significant.