Figure 2.

Fine specificity of T-cell clones and recognition of naturally processed hTERT epitopes. T-cell clones from patients C and D were tested for proliferation after stimulation with irradiated EBV-transformed cells +/− antigen. Panels (A)–(C) show mean cpm of triplicate wells. (A) Fine specificity analysis by stimulation with truncated peptides covering the GV1001 sequence (aa sequences given in right text box). The core sequence recognized by each clone is different and highlighted (red) in text boxes bellow chart. (B, C) Recognition of naturally processed antigens. T-cell clones were stimulated with EBV-transformed cells incubated with a 173 aa recombinant hTERT protein fragment. The concentration of the protein fragment was titrated as indicated.