Figure 6. Dietary emulsifiers alter epithelial cell proliferation and apoptosis in a microbiota-dependent manner.

Conventional and germfree Swiss-Webster WT mice were exposed to drinking water containing CMC or P80 (1.0%) for 13 weeks. Intestinal microbiota from conventional Swiss-Webster WT mice exposed to drinking water containing CMC or P80 (1.0%) for 13 weeks were transplanted to germfree Swiss-Webster WT mice. Analysis of (A and G) CyclinD1, (B and H) CyclinD2, (C and I) Ki67, (D and J) BCL2, (E and K) BAD and (F and L) VEGFA mRNA expression by q-RT-PCR in the colon following emulsifier treatment under germfree conditions (1^st column, A–F) and following microbiota transplantation (2^nd column, G–L).