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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Cancer Res. 2016 Nov 7;77(1):27–40. doi: 10.1158/0008-5472.CAN-16-1359

Figure 7. Dietary emulsifiers promote intestinal inflammation and carcinogenesis in the absence of DSS.

Figure 7

WT mice were exposed to drinking water containing CMC or P80 (1.0%) for 12 weeks. Mice were injected intraperitoneally with AOM (10 mg/kg body weight) weekly for a total of 7 injections. (A) Colon weights, (B) colon lengths, (C) spleen weights and (D) fat-pad mass. (E–I) Analysis of (E) IL-6, (F) CXCL1, (G) CXCL2, (H) IL-22 and (I) TNF-α mRNA expression by q-RT-PCR in colons of emulsifier-AOM treated mice. (J–L) Fecal Lcn2 concentration at day 0 (J), 56 (K) and 84 (L). (M) Representative colonoscopy from each experimental group at the end of the protocol. (N) Number of tumor per mouse and (O) total tumor surface determined using a dissecting microscope fitted with an ocular micrometer. (P–R) Analysis of (P) CyclinD1, (Q) CyclinD2 and (R) Ki67 mRNA expression by q-RT-PCR in colons of emulsifier-AOM treated mice. Data are the means +/− S.E.M. (n=10). Significance was determined using t-test (* indicates p<0.05).