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. 2017 Jan 4;12(1):e0169123. doi: 10.1371/journal.pone.0169123

Fig 8. Crosstalk between JNKs and Smad2/3 signaling pathway in response to BMP9-induced osteogenic differentiation of hPDLSCs.

Fig 8

(A) Western blotting results showed that treatment with the JNK inhibitor SP600125 at different concentrations (5, 10, or 15 mM) has a negative effect on BMP9-induced phosphorylation of Samd2/3, in a dose-dependent manner. (B) Western blotting results suggested that gene silencing of JNKs decreased the amount of p-Smadd2/3 in hPDLSCs stimulated by BMP9. (C) Western blotting results showed that BMP9 stimulated phosphorylation of JNKs and amount of Smad2/3 compared to the GFP group. The formation of protein complexes of Smad2/3 and p-JNK, detected by a co-IP assay, suggested the an interaction between Smad2/3 and p-JNK.