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. 2017 Jan 12;2(1):e89373. doi: 10.1172/jci.insight.89373

Figure 3. Thrombin generation is decreased in a protein disulfide isomerase–dependent manner following isoquercetin administration.

Figure 3

(A) Platelet-dependent thrombin generation was evaluated in platelet-rich plasma incubated with no addition (None), 10 μM isoquercetin (IsoQ), 50 μM protein disulfide isomerase (PDI), or both (IsoQ+PDI). The reaction was initiated using 0.1 U/ml thrombin and platelet-dependent thrombin generation detected using a fluorescent thrombin-specific substrate. Triplicate results and mean values are represented as peak thrombin generated (U/ml) following initiation of reaction. P values were determined by 1-way ANOVA with Sheffe’s correction for multiplicity of comparison. (B) Ten healthy participants received an oral dose of 1,000 mg isoquercetin and blood samples were drawn just before (baseline, PDI) and 4 hours after (IsoQ, IsoQ+PDI) ingestion. Samples were subsequently incubated in the absence (baseline, IsoQ) or presence (PDI, IsoQ+PDI) of PDI and assayed for platelet-dependent thrombin generation using a fluorescent thrombin-specific substrate. Top and bottom of box represent the interquartile range along with median values, whiskers represent 95th percentiles, and outliers are shown (n = 10). P values were determined using a mixed-model ANOVA with Sheffe’s correction for multiplicity of testing.