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. 2017 Jan 5;7:1994. doi: 10.3389/fpls.2016.01994

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Copyright © 2017 Vannozzi, Donnini, Vigani, Corso, Valle, Vitulo, Bonghi, Zocchi and Lucchin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Real-time RT-PCR validation of a set of genes differentially expressed in mRNA-Seq analyses in 101.14 and in an experimental tolerant rootstock (M1). A subset of DE genes was screened by qPCR upon −Fe, +Fe, and FeBic conditions both in 101.14 M1 genotypes. Transcript were normalized to the expression of elongation factor (EF1) and plotted as normalized transcript level. Bars indicate standard error (SE) in three technical replicates. Empty columns represent 101.14 genotype whereas dark gray bars represent M1. The gene considered in qPCR analyses were LDOX (VIT_06s0004g00770), FERRITIN (VIT_13s0067g01840), NODULIN (VIT_19s0093g00320), AHA2 (VIT_11s0052g00620, IRT1 (VIT_10s0042g01100), FRO2 (VIT_15s0046g01900), bHLH38/39 (VIT_08s0007g05300), POPEYE (VIT_17s0000g06000), BRUTUS (VIT_17s0000g03440).