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. 2017 Jan 5;7:40187. doi: 10.1038/srep40187

Figure 3. AMBN knockdown suppresses apoptosis, sensitivity to doxorubicin, and promotes cell migration and colony formation through the Src-Stat3 pathway in NOS-1 cells.

Figure 3

(A) The expression of AMBN, RUNX2, ALP, and OCN at the mRNA level (left panel) and AMBN, pY705-Stat3, total-Stat3, pY416-Src, total-Src and cleaved caspase-3 at the protein level in shscramble, shAMBN1, and shAMBN2 NOS-1 cells was examined (right panel). (B,F) shAMBN2 NOS-1 cells were pretreated with SU6656 or S3I-201 at indicated concentration for 24 h. The pretreated shAMBN-2 NOS-1 cells were subsequently treated with DMSO or doxorubicin (0.5 μg/mL) for 24 h and the expression of pY416-Src, total-Src, pY705-Stat3, total-Stat3, and cleaved caspase-3 was examined. (C,G) shscramble and shAMBN-2 NOS-1 cells were treated with SU6656 o S3I-201 at indicated concentration and cell growth was counted on days 0, 1, 2, and 3 (N = 3). (D,H) Cell migration activity of pretreated shscramble and shAMBN-2 NOS-1 cells was examined. Wound areas at 5 h were quantified in the graphs (N = 3). (E,I) Colony formation in pretreated shscramble and shAMBN-2 NOS-1 cells was analyzed. Quantification of colony number (left panel) and size (right panel) was shown (N = 20). Mean ± SEM (C–E,G–I); **P < 0.01; *P < 0.05.