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. Author manuscript; available in PMC: 2017 Jan 5.
Published in final edited form as: Anesthesiology. 2010 Feb;112(2):305–315. doi: 10.1097/ALN.0b013e3181ca33a1

Fig. 4.

Fig. 4

Progenitor proliferation in the dentate gyrus (DG) after 4 h of isoflurane (iso) in 16-month-old rats. Bromodeoxyuridine (BrdU) (50 mg/kg) was injected twice with a 12-h interval 4 days after 4 h of isoflurane (n = 6) or no isoflurane (control, n = 6). Immunocytochemical staining of bromodeoxyuridine+ cells (arrows in A, main image 4×, inset 20× objective) of every twelfth coronal section of the hippocampus and quantification of every bromodeoxyuridine+ cell (B) revealed no difference between groups (P > 0.2, Mann–Whitney U test).