Table 1.

Algorithm for Changing Isoflurane Concentration in Response to Tail Clamping

Number of Animals Moving in Response to Tail Clamping	Change in Isoflurane Concentration, %
	First Hour	Second–Fourth Hours
0	−0.8	−0.6
1	−0.5	−0.4
2	−0.3	−0.3
3	−0.2	−0.2
4	−0.1	−0.1
5	No change	No change
6	No change	−0.1
7	−0.1	−0.2
8	−0.2	−0.3
9	−0.4	−0.4
10	−0.6	−0.6