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. 2017 Jan 4;37(1):152–163. doi: 10.1523/JNEUROSCI.1698-16.2016

Figure 1.

Figure 1.

The large majority of Aβ in brain extracts elutes in or near the void volumes upon SEC using Superdex 75 and Superdex 200. A, Human brain extracts (200 μl) were chromatographed on a Superdex 75 column, fractions collected, lyophilized, and used for Western blotting with a mix of 2G3, 21F12, and 6E10. The major Aβ-immunoreactive species detected in all 4 AD brain extracts migrated on SDS-PAGE at ∼4 and ∼7.5 kDa and were most abundant in SEC fractions within or near the void volume of the Superdex 75 column. B, The elution of Aβ species on Superdex 75 versus Superdex 200 was compared by chromatographing aliquots of the same AD sample on the 2 different columns. The majority of the ∼4 and ∼7.5 kDa species eluted from both the Superdex 75 and Superdex 200 columns in or near the void. A, B, A ladder of MW standards is shown on the left of each gel. Arrows indicate the 14, 6, and 3 kDa standards. The SEC fraction numbers and the position and quantity of 1 ng, 5 ng synthetic Aβ1–40 standards are indicated at the top of the gels. Arrow indicates the elution of a 680 kDa marker protein from SEC.