Peripheral CGRP does not induce anxiety in the open-field assay. A, Time spent in the center of the open field after intraperitoneal injection of vehicle (n = 10) or CGRP (0.1 mg/kg, n = 10) or in CD1 mice over the 30 min testing period (left). Right panel shows the mean percentage time (±SEM) in center per 5 min interval for individual mice. B, Time spent in the center of the open field after intraperitoneal injection of vehicle (n = 9) or CGRP (0.1 mg/kg, n = 9) or in C57BL/6J mice over the 30 min testing period (left). Right panel shows the mean percentage time (±SEM) in center per 5 min interval for individual mice on treatment day. In both A and B, there were no significant differences.