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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Brain Behav Immun. 2016 Nov 7;60:282–292. doi: 10.1016/j.bbi.2016.11.003

Figure 6. The algesic MBP69-86 peptides induce MMPs in nerve.

Figure 6

A, von Frey testing after single bolus i.n. injection of MBP69-86 and MBP2-18 peptides (50 μg in 5 μl, each) into a naïve sciatic nerve. A sustained decline in mechanical withdrawal thresholds was observed for two weeks after the MBP68-86 injection but not following the MBP2-18 injection. No change in thresholds was observed in the contralateral hind paws of either group. The mean withdrawal thresholds (gram force; g) ± SEM of n=6/group. *, p≤0.05, **, p≤0.01, by one-way ANOVA with Bonferroni’s corrections. B, Taqman qRT-PCR for MT1-MMP, MMP-2 and MMP-9 in nerve after A. (day 14 post-injection). The mean relative mRNA ± SEM of n=4–6 duplicate samples per group normalized to GAPDH and calibrated to naïve nerve. *, p≤0.05, **, p≤0.01, ***, p≤0.001, n.s, not significant, by one-way ANOVA with Tukey’s post-hoc test.