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Relative RNA-binding affinity by RNA-binding factors in RNA immunoprecipitation.
| Factor | Region in MOR mRNA | Relative RNA-binding affinitya |
|---|---|---|
| PCBP1 (PB1) | 5'-UTR | − |
| E3/4 | ++ | |
| RPL32 | +++ | |
| 3′-UTR repeat | − | |
| CA repeat 2 | − | |
| 3′-UTR end | − | |
| hnRNPK (hRK) | 5'-UTR | ++++ |
| E3/4 | ++ | |
| RPL32 | +++ | |
| 3′-UTR repeat | ++ | |
| CA repeat 2 | + | |
| 3′-UTR end | +/− |
Relative binding affinity was measured by setting the highest (++++) level with hnRNPK's binding in 5′-UTR region and the lowest level (−) with gal4's binding in 5′-UTR region.
