Table 1.

List of primers (restriction sites were showed in bold)

Primers	Sequence 5′to 3′	Orientation	5′ cloning site
A) Primers used for amplification of cd52 gene
CD52 F	5′-AACTGCAGGGCCACCATGAAGCGCTTCCTC-3	Sense	PstI
CD52 R	5′-CCTCTAGATCAACTGAAGCAGAAGAG-3′	Anti-sense	XbaI
B) Primers used for Real time PCR
RT CD52 F	5′- TTCCTCTTCCTCCTACTCACC-3′	Sense	-
RT CD52 R	5′-ATGCCTCCGCTTATGTTGCT-3′	Anti-sense	-
CHO b2m F	5′-TGTTGAAGAATGGAAAGAAGATGGA-3′	Sense	-
CHO b2m R	5′-CGGTCGCAGTGGGTGTAAA-3′	Anti-sense	-
C) Universal primers
T7f	5′-GTA AAA CGA CGG CCA GT	Sense	-
pBudCE4.1r	5′-CAG GAA ACA GCT ATG AC	Anti-sense	-