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. 2017 Jan 5;7:39711. doi: 10.1038/srep39711

Figure 2. Expression of NPR2 on sperm flagellum.

Figure 2

(a) Capacitated spermatozoa were incubated with FAM-NPPC (100 nM) for 30 min to ensure fully reaction. The binding of FAM-NPPC to the spermatozoon as visualized by fluorescence (up panel) and merge (down panel) fields. Scale bar, 20 μm. (b) Comparison of FAM-NPPC binding in spermatozoa without or with NPPC (1 μM) competition. Bars indicate the mean ± SEM of three experiments. n = 500 for each group. **P < 0.01. (c) A representative western blot of NPR2 from sperm heads and flagella. NPR2 antibody recognized two immunoreactive bands that were obvious in the fraction of sperm flagella.