Table 2.

Comparison between RT-LAMP and nested RT-PCR using clinical samples

Sample number	Breakpoint type	RNA concentration (ng/μL)	Absorbance ratio (260/280 mm)	RIN value	RT-LAMP	Nested RT-PCR
S-1	bcr1	250.8	2.04	9.60	+	+
S-2	bcr1	535.0	2.04	10	+	+
S-3	bcr1	361.9	1.96	NM	–	–
S-4	bcr1	1141.0	2.06	NA	+	+
S-5	bcr3	389.8	2.06	9.50	+	+
S-6	bcr3	309.2	2.05	9.90	+	+
S-7	bcr3	59.9	2.04	8.50	+	+

NA, not available; NM, not measured due to pattern deformation; RIN, RNA integrity number; RT-LAMP, reverse transcription loop-mediated isothermal amplification; RT-PCR, reverse transcription polymerase chain reaction; Samples 5 and 6 were derived from the same patient: S-5, at the initial visit and S-6, at the time of relapse.