CXCR4, CXCR7, and CXCL12 expression in CRLMs. Representative microphotograph (200× magnification) for CXCR4, CXCR7 and CXCL12 IHC staining in CRLM as (A, B) Diffuse low and moderate and (C) diffuse high cytoplasmic and/or nuclear staining for CXCR4: Arrow shows area with staining in stromal cells of microenvironment. (D, E) Negative/low, and (F) high CXCR7 cytoplasmic staining. Arrow shows staining in stromal cells of microenvironment. (G) Negative low and (H, I) high membrane and cytoplasmic CXCL12 staining.