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PD-L1 and PD-1 protein expression. Representative microphotograph for PD-1 and PD-L1 IHC staining in CRLM (200× magnification). (A) Negative and (B) focal PD-1 immunoreactivity on microenvironment immune cells at tumor periphery (inset detail: 400× magnification). (C) Negative or (D) diffuse PD-L1 membrane with or without cytoplasmic staining on tumor and stromal cells (inset : 400× magnification).
