Figure 5.
Electron photomicrographs showing dual labeling for DOPR and CRF in the BLA and CeA. A. A DOPR and CRF-labeled dendrite (DOPR+CRF-d) receives a symmetric synaptic contact (arrowheads) from an unlabeled terminal (ut) in the BLA. B. A DOPR+CRF-d receives an asymmetric synapse (zigzag arrow) from an unlabeled terminal (ut) in the BLA. C. A DOPR-labeled axon terminal (DOPR-t) in the BLA forms a symmetric synapse (arrowheads) with a CRF-labeled dendrite (CRF-d). D. A DOPR and CRF-labeled axon terminal (DOPR+CRF-t) contacting (arrowheads) an unlabeled dendrite (ud) in the BLA. E. An unlabeled axon terminal (ut) is contacting a DOPR+CRF-d in the CeA. F. A DOPR+CRF-d receives a symmetric synapse (arrowheads) from an unlabeled terminal (ut). G. A DOPR-labeled axon terminal (DOPR-t) in the CeA contacting a CRF-d. H. A DOPR and CRF-labeled axon terminal (DOPR+CRF-t) forming a symmetric synapse (arrowheads) with an unlabeled dendrite (ud) in the CeA. Arrows point to DOPR-immunogold silver particles while double arrowheads point to peroxidase immunolabeling indicative of CRF. ut, unlabeled terminal. Scale bar, 0.50μm.