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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Brain Struct Funct. 2016 Jul 4;222(2):1007–1026. doi: 10.1007/s00429-016-1261-6

Figure 7.

Figure 7

High magnification confocal immunofluorescence photomicrographs demonstrating DOPR (A,E), CRF (B,F) and NET (C,G) immunoreactivities in the coronal section through the BLA (A–D) and CeA (E–H). DOPR immunoreactivity was labeled with fluorescein isothiocyanate (green), CRF was labeled with rhodamine isothiocyanate and pseudocolored with blue, and NET was labeled with cyanine dye 5 (Cy5) and pseudocolored with red. We pseudocolored NET with red to allow easier identification of co-localized DOPR and NET which produces yellow color (arrowheads). The merged images illustrate DOPR and CRF-containing neurons that are in close proximity to NET-labeled fibers (D,H). Arrows illustrate neurons that co-express DOPR and CRF.. Thick arrows indicate single labeled DOPR, CRF or NET. Arrows indicate dorsal (D) and lateral (L) orientation. Scale bar, 25 μm.