Table 3.
Positive-negative selection.
| Exp.(A) | Vector | Number of immature embryos in P-N selection on hygromycin(B) | Transformation efficiency in pMBVec10 control(C) | Number of independent transformation events predicted in P-N selection(D) | GT(E) | ||||
|---|---|---|---|---|---|---|---|---|---|
| 1 | pINA-ALSmS629N | 180 | 100% | 180 | 0 | ||||
| 2 | pINA-ALSmS629N | 284 | 58% | 164 | 0 | ||||
| 3 | pINA-ALSmS629N | 391 | 61% | 239 | 0 | ||||
| 4 | pINA-ALSmS629N | 280 | 100% | 280 | 0 | ||||
| 5 | pINA-ALSmS629N | 280 | 90% | 252 | 0 | ||||
| 6 | pINA-ALSmS629N | 230 | 68% | 155 | 0 | ||||
| Total | 1645 | 1270 | 0 | ||||||
(A) Experiment number. (B) Immature embryos prepared from wild type were transformed with the P-N targeting vector pINA-ALSmS629N and selected on hygromycin. (C) In the reference transformation, 20 immature embryos were transformed in the same experiment with pMBVec10 and selected on hygromycin. The transformation efficiency in the reference was calculated by dividing the number of embryos forming callus in the presence of hygromycin by the number of embryos transformed. (D) The number of independent transformants obtained by P-N selection was obtained by multiplication of the transformation efficiency obtained with pMBVec10 (C) with the number of immature embryos in the P-N selection (B). (E) The number of gene targeting (GT) events obtained.