FIG 1.
KLF4 and Krox20 are dispensable for induction of early adipogenic TFs. (A, B) 3T3-L1 white preadipocytes (A) and Pparγf/f immortalized brown preadipocytes (B) were induced to undergo adipogenesis. Cells were collected at the indicated time points for RNA-Seq. RPKM values from RNA-Seq indicate gene expression levels. D2, day 2; D7, day 7. (C, D) Klf4f/f (C) and Krox20f/f (D) immortalized brown preadipocytes were infected with retroviral vector MSCVhygro expressing Cre. Adipogenesis was induced with the adipogenic cocktail, and cells were collected at indicated time points for qRT-PCR analysis of Klf4, Krox20, Cebpβ, Cebpδ, Klf5, and Klf9.