FIG 3.
KLF4 is dispensable for white adipogenesis in culture. (A, B) Klf4f/f primary white preadipocytes were infected with Ad-GFP or Ad-Cre, followed by adipogenesis assays. (A) Oil Red O staining of differentiated cells at D10 (day 10). (B) qRT-PCR analysis of gene expression. (C to E) 3T3-L1 white preadipocytes were infected with lentiviral vector expressing control (shControl) or KLF4 knockdown shRNA (shKLF4), followed by adipogenesis assays. (C) Western blot analysis of KLF4 in preadipocytes. The asterisk indicates a nonspecific band. (D) Oil Red O staining of differentiated cells at D7. (E) qRT-PCR analysis of gene expression.