Fig. 5. Effects of atractylodin on myosin phosphorylation-related mechanisms.

Panel A: phosphorylation extent (a1) and statistical analysis (a2) of 20-kDa myosin light chain (p-MLC₂₀), Panel B: protein contents (b1) and statistical analysis (b2) of myosin light chain kinase (MLCK), Panel C: MLCK mRNA expression in jejunal segment in normal control (NC) rats, atractylodin-treated CP rats (CP+Atrac), and atractylodin-treated DP rats (DP+Atrac). Data were expressed as the mean±SD from four independent experiments; ^**p<0.01 compared with the normal controls; ^##p<0.01 compared with CP group or DP group before being given atractylodin, respectively. p-MLC₂₀, phosphorylated 20-kDa myosin light chain; MLC₂₀, 20-kDa myosin light chain; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.