Fig. 4. KLF8 suppresses promoter activity of KLF5.

(A) Contractile type of VSMCs were stimulated with TNFα for the indicated times. Expression of KLF5 and contractile marker proteins were verified by western blot analysis. (B, C) Promoter activity of both KLF5 and KLF4 was measured in the presence of KLF8. (D, E) Promoter activity of SMA and SM22α was measured in the presence of KLF5. (F) KLF5 was overexpressed in contractile type of VSMCs, and AngII-induced contraction of VSMCs was measured as described in the “Materials and Methods” section. Data are expressed as the mean±S.D. for three independent experiments (n=3 for each experiment). ^*Significantly different from values of vector group (p<0.05).