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. 2017 Jan 12;2(1):e91419. doi: 10.1172/jci.insight.91419

Figure 1. PPARD promotes lung metastases of B16-F10 melanoma cells in immunocompetent mice.

Figure 1

(A–F) WT B16-F10 melanoma cells or B16-F10 melanoma cells stably transduced with PPARD-shRNA-A (PPARD-shRNxA-A-clone1 or -clone2) or control-shRNA plasmid, or with two independent PPARD-shRNA (PPARD-shRNA-C or -D) or control-shRNA lentivirus, were injected via the tail vein into syngeneic C57BL/6 mice, and lung metastasis formation was evaluated 3 weeks later. (A) Representative photographs of Bouin-fixed lung specimens of mice injected with plasmid-transfected cells. (B) Numbers of lung metastases per mouse for each group (n = 12–14 per group) shown in A. (C) Representative photographs of freshly isolated lungs in a repeat experiment. (D) Numbers of lung metastases per mouse for each group (n = 8 per group) shown in C. (E) Representative photographs of formalin-fixed lung specimens of mice injected with lentivirus-tranduced cells. (F) Numbers of lung metastases per mouse for each group (n = 6 per group) shown in E. All scale bars: 1 cm. Values in B, D, and F are mean ± SEM. Lines indicate means. ***P < 0.0001 (2-sided Poisson).