Table 2.
Summary of binding affinity (KD, nM) for the interaction of all test compounds with biotin labeled DNA sequences using biosensor-SPR method [a]
| DB 2429 |
DB 2464 |
DB 2432 |
DB 847 |
DB 1300 |
DB 2457 |
DB 2430 |
DB 2465 |
|
|---|---|---|---|---|---|---|---|---|
| AAA TTT |
524 | 1160 | NB | 731 | 277 | 222 | 74 | 330 |
| AAA G TTT |
50 | 70 | 303 | 559 | 196 | 4 | 180 | 690 |
| AAA GC TTT |
1086 | 1060 | NB | NB | 708 | 192 | 360 | 1400 |
| AAA I TTT |
322 | 883 | -- | -- | -- | 322 | -- | -- |
| ATA G TAT |
432 | 590 | -- | -- | -- | 297 | -- | -- |
| AATT G AATT |
983 | 1493 | -- | -- | -- | 226 | -- | -- |
[a]
All the results in this table were investigated in Tris-HCl buffer (50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 0.05% P20, pH 7.4) at a 100 µL/min flow rate. “--” experiment not done, “NB” no binding. The listed binding affinities are an average of two independent experiments carried out with two different sensor chips and the values are reproducible within 10% experimental errors.