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. Author manuscript; available in PMC: 2017 Jan 5.
Published in final edited form as: Microbiology (Reading). 2014 Jan 21;160(Pt 3):616–622. doi: 10.1099/mic.0.074807-0

Figure 3.

Figure 3

2DE analysis of metronidazole treated 638R.

(a), integrity and relative abundance of NimA and the putative reductase (E1WVQ6_BACF6) were checked on 2D-gels after 2 h of incubation of equally divided 638R (nimA) stationary phase cultures either without drug or with 50 µM of metronidazole. The broken circle indicates the location of the metronidazole adduct of the isolated protein spot. Percentages refer to the relative abundance of the measured proteins as compared to total protein visualized.

(b), validation of metronidazole adduct formation in 638R (nim-negative). Broken circles indicate adducts of the metronidazole adduct of the isolated protein spot with either metronidazole or tinidazole.